Human Actin, gamma-enteric smooth muscle (ACTG2) ELISA Kit

Actin, gamma-enteric smooth muscle is a protein encoded by the ACTG2 gene.Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 2, encoded by this gene, is a smooth muscle actin found in enteric tissues.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Actin, gamma-enteric smooth muscle (ACTG2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACTG2. No significant cross-reactivity or interference between Human ACTG2 and analogues was observed.

This Human Actin, gamma-enteric smooth muscle (ACTG2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTG2 in samples. An antibody specific for ACTG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.