Human β-actin ELISA Kit

Beta-actin (gene name ACTB) is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. In a neoplastic cell line resulting from treatment of cultured human diploid fibroblasts with a chemical mutagen, Leavitt et al. (1982) observed a mutant form of beta actin. Toyama and Toyama (1984) isolated and characterized lines of KB cells resistant to cytochalasin B. They found that one resistant line had an alteration in beta-actin. Such cells bound less cytochalasin B than did parental KB cells. The authors suggested that the primary site of action of cytochalasin B on cell motility processes is beta-actin.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human β-actin ELISA Kit has high sensitivity and excellent specificity for detection of Human ACTB. No significant cross-reactivity or interference between Human ACTB and analogues was observed.

This Human β-actin ELISA Kit employs a two-site sandwich ELISA to quantitate ACTB in samples. An antibody specific for ACTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTB bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.