Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) ELISA Kit

ACAT2 is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.As the human TCP1 gene had been assigned to 6q25-q27 by study of somatic cell hybrids and by in situ hybridization, the ACAT2 gene was suspected to be localized to the same chromosome region. Retention of this close linkage during mammalian evolution suggests the possibility of some functional significance. Transcription of both DNA strands at a given locus is common in prokaryotic and viral systems. For examples of overlapping transcriptional units in humans.

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Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACAT2. No significant cross-reactivity or interference between Human ACAT2 and analogues was observed.

This Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACAT2 in samples. An antibody specific for ACAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.