Human Retinal-specific ATP-binding cassette transporter (ABCA4) ELISA Kit

ABCA4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies. This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is a retina-specific ABC transporter with N-retinylidene-PE as a substrate. It is expressed exclusively in retina photoreceptor cells, indicating the gene product mediates transport of an essental molecule across the photoreceptor cell membrane. Mutations in this gene are found in patients diagnosed with Stargardt disease, a form of juvenile-onset macular degeneration.

ABCA4; ABC10; ABCR; ARMD2; CORD3; DKFZp781N1972; FFM; FLJ17534; RMP; RP19; STGD; STGD1; ATP binding cassette transporter; ATP-binding cassette; sub-family A member 4; ATP-binding transporter; retina-specific

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Human Retinal-specific ATP-binding cassette transporter (ABCA4) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCA4. No significant cross-reactivity or interference between Human ABCA4 and analogues was observed.

This Human Retinal-specific ATP-binding cassette transporter (ABCA4) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCA4 in samples. An antibody specific for ABCA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ABCA4; ABC10; ABCR; ARMD2; CORD3; DKFZp781N1972; FFM; FLJ17534; RMP; RP19; STGD; STGD1; ATP binding cassette transporter; ATP-binding cassette; sub-family A member 4; ATP-binding transporter; retina-specific

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.