Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (AADAT) ELISA Kit

Aminoadipate Aminotransferase is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist. Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.

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Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (AADAT) ELISA Kit has high sensitivity and excellent specificity for detection of Human AADAT. No significant cross-reactivity or interference between Human AADAT and analogues was observed.

This Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (AADAT) ELISA Kit employs a two-site sandwich ELISA to quantitate AADAT in samples. An antibody specific for AADAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAADAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AADAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AADAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.