Human Septin-8 (SEPT8) ELISA Kit

SEPT8 is a member of the highly conserved septin family. Septins are 40- to 60-kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking. A conserved region found in all 3 variants contains the GTP-binding motif and is encoded by exons 3 through 10. Northern blot analysis detected expression of most SEPT8 variants in all brain and cardiovascular regions tested, as well as in prostate, testis, and ovary. Expression was highest in brain and aorta, and expression of each variant was tissue specific. Western blot analysis detected variant 1 at an apparent molecular mass of 58 kD in prostate, testis, and ovary.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Septin-8 (SEPT8) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEPT8. No significant cross-reactivity or interference between Human SEPT8 and analogues was observed.

This Human Septin-8 (SEPT8) ELISA Kit employs a two-site sandwich ELISA to quantitate SEPT8 in samples. An antibody specific for SEPT8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEPT8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEPT8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEPT8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.