Human RNA-binding protein 8A (RBM8A) ELISA Kit

RBM8 encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.

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Human RNA-binding protein 8A (RBM8A) ELISA Kit has high sensitivity and excellent specificity for detection of Human RBM8A. No significant cross-reactivity or interference between Human RBM8A and analogues was observed.

This Human RNA-binding protein 8A (RBM8A) ELISA Kit employs a two-site sandwich ELISA to quantitate RBM8A in samples. An antibody specific for RBM8A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBM8A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBM8A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBM8A bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.