Human Retinol-binding protein 3 (RBP3) ELISA Kit

Retinol-binding protein 3, interstitial (RBP3), also known as IRBP is a protein identified in most eutherians except tenrecs and armadillos.The interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process. The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da.

RBP3; D10S64; D10S65; D10S66; IRBP; RBPI; interphotoreceptor retinoid-binding protein; retinol-binding protein 3; retinol-binding protein 3; interstitial

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Retinol-binding protein 3 (RBP3) ELISA Kit has high sensitivity and excellent specificity for detection of Human RBP3. No significant cross-reactivity or interference between Human RBP3 and analogues was observed.

This Human Retinol-binding protein 3 (RBP3) ELISA Kit employs a two-site sandwich ELISA to quantitate RBP3 in samples. An antibody specific for RBP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

RBP3; D10S64; D10S65; D10S66; IRBP; RBPI; interphotoreceptor retinoid-binding protein; retinol-binding protein 3; retinol-binding protein 3; interstitial

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.