Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit

Sequence analysis predicted that GAIP is a 24.6-kD, hydrophilic protein that lacks a transmembrane domain and contains multiple potential phosphorylation sites. GAIP shares approximately 31% amino acid identity with regulator of G protein signaling-1 (RGS1) and -2 (RGS2) ; the 125-amino acid core domains of GAIP, RGS1, and RGS2 share approximately 64% homology. Northern blot analysis detected a 1.6-kb GAIP transcript in lung, placenta, liver, heart, and pancreas, with almost no expression detected in brain, skeletal muscle, and kidney. Yeast 2-hybrid binding analyses showed that through its core domain, GAIP interacts strongly with GNAI3 and weakly with GNAI2, but does not interact with GNA11.

RGS19; GAIP; RGSGAIP; G alpha interacting protein; G protein signalling regulator 19; guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein; regulator of G-protein sig

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Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit has high sensitivity and excellent specificity for detection of Human RGS19. No significant cross-reactivity or interference between Human RGS19 and analogues was observed.

This Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit employs a two-site sandwich ELISA to quantitate RGS19 in samples. An antibody specific for RGS19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGS19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGS19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS19 bound in the initial step. The color development is stopped and the intensity of the color is measured.

RGS19; GAIP; RGSGAIP; G alpha interacting protein; G protein signalling regulator 19; guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein; regulator of G-protein sig

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.