Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit

The deduced 469-amino acid protein has the characteristic 7-transmembrane domain structure of a G protein-coupled receptor, as well as 2 putative N-glycosylation sites in its N-terminal domain. SALPR shares highest sequence similarity with somatostatin receptors and angiotensin receptors. SALPR expression was not detected by Northern blot analysis. RT-PCR detected SALPR expression predominantly in brain, with highest expression in substantia nigra and pituitary, followed by hippocampus, spinal cord, amygdala, caudate nucleus, and corpus callosum; expression was very low in cerebellum. In peripheral tissues, relatively high expression was detected in adrenal gland, and low expression was detected in pancreas, salivary gland, placenta, mammary gland, and testis. Little to no expression was detected in other tissues examined.

RXFP3; GPCR135; MGC141998; MGC142000; RLN3R1; RXFPR3; SALPR; G-protein coupled receptor SALPR; relaxin 3 receptor 1; relaxin family peptide receptor 3; somatostatin and angiotensin-like peptide receptor

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit has high sensitivity and excellent specificity for detection of Human RXFP3. No significant cross-reactivity or interference between Human RXFP3 and analogues was observed.

This Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit employs a two-site sandwich ELISA to quantitate RXFP3 in samples. An antibody specific for RXFP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRXFP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RXFP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RXFP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

RXFP3; GPCR135; MGC141998; MGC142000; RLN3R1; RXFPR3; SALPR; G-protein coupled receptor SALPR; relaxin 3 receptor 1; relaxin family peptide receptor 3; somatostatin and angiotensin-like peptide receptor

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.