Human Retinoic acid receptor RXR-alpha (RXRA) ELISA Kit

Retinoid X receptor alpha (RXR-alpha), is a nuclear receptor.Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcription factors. This receptor differs from the other 3 RARs within the ligand-binding domain and is incapable of high affinity binding of retinoic acid itself. The retinoic acid, thyroid hormone, and vitamin D receptors, as well as the retinoid X receptor, activate transcription from response elements containing 2 or more degenerate copies of the consensus motif AGGTCA.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Retinoic acid receptor RXR-alpha (RXRA) ELISA Kit has high sensitivity and excellent specificity for detection of Human RXRA. No significant cross-reactivity or interference between Human RXRA and analogues was observed.

This Human Retinoic acid receptor RXR-alpha (RXRA) ELISA Kit employs a two-site sandwich ELISA to quantitate RXRA in samples. An antibody specific for RXRA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRXRA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RXRA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RXRA bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.