Human Soluble protein-100 (S-100) ELISA Kit

A1is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. S100A1 overexpression enhances cardiac contractile performance which suggests that S100A1 is a regulator of myocardial contractility. S100A1 improves cardiac performance both by regulating calcium ion handling by the sarcoplasmic reticulum and the responsiveness of myofibrils to calcium ion.

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Human Soluble protein-100 (S-100) ELISA Kit has high sensitivity and excellent specificity for detection of Human S100A1. No significant cross-reactivity or interference between Human S100A1 and analogues was observed.

This Human Soluble protein-100 (S-100) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A1 in samples. An antibody specific for S100A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.