Human Protein S100-A7 (S100A7) ELISA Kit

S100 calcium binding protein A7 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The protein functions as a prominent antimicrobial peptide mainly against E. coli.

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Human Protein S100-A7 (S100A7) ELISA Kit has high sensitivity and excellent specificity for detection of Human S100A7. No significant cross-reactivity or interference between Human S100A7 and analogues was observed.

This Human Protein S100-A7 (S100A7) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A7 in samples. An antibody specific for S100A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.