Human Amiloride-sensitive sodium channel subunit gamma (SCNN1G) ELISA Kit

The epithelial sodium channel (short: ENaC, also: sodium channel non-neuronal 1 (SCNN1) or amiloride sensitive sodium channel (ASSC) ) is a membrane-bound ion-channel that is permeable for Li+-ions, protons and especially Na+-ions. It is a constitutively active ion-channel. It is arguably the most selective ion channel. Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. Amiloride Sensitive Sodium Channel Subunit Gammaisthe gamma subunit, and mutations in this gene have been associated with Liddle syndrome..

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Human Amiloride-sensitive sodium channel subunit gamma (SCNN1G) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCNN1G. No significant cross-reactivity or interference between Human SCNN1G and analogues was observed.

This Human Amiloride-sensitive sodium channel subunit gamma (SCNN1G) ELISA Kit employs a two-site sandwich ELISA to quantitate SCNN1G in samples. An antibody specific for SCNN1G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCNN1G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCNN1G is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCNN1G bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.