Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit

By real-time PCR, Schulze-Tanzil et al. (2004) found that expression of SCX, a tendon-specific marker, increased in human tenocytes during their entire time in high-density cultures.By in situ hybridization, Brent et al. (2003) found that Scx was expressed in axial and ventrolateral body wall tendons during chicken embryonic development. It was also expressed in a population of tendon progenitors in developing somites. Brent et al. (2003) determined that FGF signaling in the myotome was both necessary and sufficient for induction of Scx expression in developing chicken somites. Overexpression of Pax1 inhibited Scx expression in somites.Hartz (2004) mapped the SCX gene to chromosome 8q24.3 based on an alignment of the SCX sequence with the genomic sequence.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCXA. No significant cross-reactivity or interference between Human SCXA and analogues was observed.

This Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit employs a two-site sandwich ELISA to quantitate SCXA in samples. An antibody specific for SCXA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCXA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCXA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCXA bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.