Human L-Selectin (SELL) ELISA Kit

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. It is cleaved by ADAM17.The protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. L-selectin acts as a "homing receptor" for leukocytes to enter tissues at the site of inflammation. Ligands present on endothelial cells will bind to leukocytes expressing L-selectin, causing them to become localised at that point. The receptor is also found on the cell surfaces of "naive" T cells, which have not yet encountered their specific antigen. This surface expression is lost after the cells are activated.

SELL; CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; leukocyte surface antigen Leu-8; leukocyte-endothelial cell adhesion molecule 1; lymph node homing

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human L-Selectin (SELL) ELISA Kit has high sensitivity and excellent specificity for detection of Human SELL. No significant cross-reactivity or interference between Human SELL and analogues was observed.

This Human L-Selectin (SELL) ELISA Kit employs a two-site sandwich ELISA to quantitate SELL in samples. An antibody specific for SELL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELL bound in the initial step. The color development is stopped and the intensity of the color is measured.

SELL; CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; leukocyte surface antigen Leu-8; leukocyte-endothelial cell adhesion molecule 1; lymph node homing

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.