Human Semaphorin-3E (SEMA3E) ELISA Kit

SEMA3E encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.The deduced 775-amino acid protein has a calculated molecular mass of about 95 kD. SEMA3E shares 87.4% identity with mouse Sema3e. RT-PCR detected highest expression in brain and prostate, followed by small intestine, thymus, and lung. Little to no expression was detected in other tissues examined.Sema3e is highly expressed in developing somites, where it acts as a repulsive cue for plexin-D1-expressing endothelial cells of adjacent intersomitic vessels. Sema3e-plexin-D1 signaling did not require neuropilins, which had been presumed to be obligate Sema3 coreceptors.

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Human Semaphorin-3E (SEMA3E) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA3E. No significant cross-reactivity or interference between Human SEMA3E and analogues was observed.

This Human Semaphorin-3E (SEMA3E) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3E in samples. An antibody specific for SEMA3E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3E bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.