Human Semaphorin 3F (S3F) ELISA Kit

The semaphorins are a family of proteins that are involved in signaling. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62), a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.

SEMA3F; SEMA-IV; SEMA4; SEMAK; sema IV; sema domain; immunoglobulin domain (Ig) ; short basic domain; secreted; 3F; semaphorin 3F; semaphorinI/F; semaphorin IV

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Human Semaphorin 3F (S3F) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA3F. No significant cross-reactivity or interference between Human SEMA3F and analogues was observed.

This Human Semaphorin 3F (S3F) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3F in samples. An antibody specific for SEMA3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3F bound in the initial step. The color development is stopped and the intensity of the color is measured.

SEMA3F; SEMA-IV; SEMA4; SEMAK; sema IV; sema domain; immunoglobulin domain (Ig) ; short basic domain; secreted; 3F; semaphorin 3F; semaphorinI/F; semaphorin IV

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.