Human Protein Z-dependent protease inhibitor (SERPINA10) ELISA Kit

Protein Z-dependent protease inhibitor is a protein circulating in the blood which inhibits factors Xa and XIa of the coagulation cascade. It is a member of the class of the serine protease inhibitors (serpins) . Its name implies that it requires protein Z, another circulating protein, to function properly, but this only applies to its inhibition of factor X. It is about 72 kDa heavy and 444 amino acids large. It is produced by the liver.Water et al. found deficiency of ZPI in 4.4% of a cohort of patients with thrombophilia (a tendency to thrombosis) .Han et al. first described ZPI in 1998. The same group further characterised it in 2000.

SERPINA10; PZI; ZPI; protein Z-dependent protease inhibitor; protein Z-dependent protease inhibitorprecursor; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin) ; member 10

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Human Protein Z-dependent protease inhibitor (SERPINA10) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINA10. No significant cross-reactivity or interference between Human SERPINA10 and analogues was observed.

This Human Protein Z-dependent protease inhibitor (SERPINA10) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA10 in samples. An antibody specific for SERPINA10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

SERPINA10; PZI; ZPI; protein Z-dependent protease inhibitor; protein Z-dependent protease inhibitorprecursor; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin) ; member 10

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.