Human Corticosteroid Binding Globulin (CBG) ELISA Kit

Transcortin is produced by the liver and is regulated by estrogens. Therefore, plasma transcortin levels increase during pregnancy, and are decreased in cirrhosis.Transcortin is produced by the liver and is regulated by estrogens. Therefore, plasma transcortin levels increase during pregnancy, and are decreased in cirrhosis. Corticosteroid-binding globulin is an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events.

SERPINA6; CBG; corticosteroid binding globulin; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin) ; member 6; transcortin

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Human Corticosteroid Binding Globulin (CBG) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINA6. No significant cross-reactivity or interference between Human SERPINA6 and analogues was observed.

This Human Corticosteroid Binding Globulin (CBG) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA6 in samples. An antibody specific for SERPINA6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

SERPINA6; CBG; corticosteroid binding globulin; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin) ; member 6; transcortin

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.