Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit

Monocyte/neutrophil elastase inhibitor (EI) is a protein of approximately 42,000 Mr with serpin-like functional properties. Remold-O'Donnell et al. (1992) cloned EI cDNA and identified 3 EI mRNA species of 1.5, 1.9, and 2.6 kb in monocyte-like cells and no hybridizing mRNA in lymphoblastoid cells lacking detectable EI enzymatic activity. The cDNA open reading frame encoded a 379-amino acid protein. Its sequence established EI as a member of the serpin superfamily. Sequence alignment indicated that the reactive center P1 residue is cys-344, consistent with abrogation of elastase inhibitory activity by iodoacetamide and making EI a naturally occurring cys-serpin. Other elastase inhibitors include alpha-1-antitrypsin (PI) and secretory leukocyte protease inhibitor (SLPI) .

SERPINB1; EI; ELANH2; LEI; M/NEI; MNEI; PI2; anti-elastase; protease inhibitor 2 (anti-elastase) ; monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin) ; member 1

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINB1. No significant cross-reactivity or interference between Human SERPINB1 and analogues was observed.

This Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINB1 in samples. An antibody specific for SERPINB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

SERPINB1; EI; ELANH2; LEI; M/NEI; MNEI; PI2; anti-elastase; protease inhibitor 2 (anti-elastase) ; monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin) ; member 1

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.