Human Serpin B5 (SERPINB5) ELISA Kit

Maspin is a serpin and tumor suppressor gene.It has been associated with prostate cancer and breast cancer.It has also been associated with tumors of the pancreas.It was identified in 1994.In normal cells expressing SERPINB5, the SERPINB5 promoter is unmethylated and the promoter region has acetylated histones and an accessible chromatin structure. In contrast, normal cells that do not express SERPINB5 have a completely methylated SERPINB5 promoter with hypoacetylated histones, an inaccessible chromatin structure, and a transcriptional repression that is relieved by inhibition of DNA methylation. These findings indicated that cytosine methylation is important in the establishment and maintenance of cell type-restricted gene expression.

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Human Serpin B5 (SERPINB5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINB5. No significant cross-reactivity or interference between Human SERPINB5 and analogues was observed.

This Human Serpin B5 (SERPINB5) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINB5 in samples. An antibody specific for SERPINB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.