Human Sestrin-2 (SESN2) ELISA Kit

Sestrin-2 is a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.The full-length SEST2 cDNA encodes a deduced 480-amino acid protein predicted to be a compact globular domain protein composed predominantly of alpha-helical structures. SEST2 has several potential serine-threonine and tyrosine phosphorylation sites, most of them located within the alpha helices. Northern blot analysis revealed a 3.9-kb transcript expressed at low to moderate levels in most tissues examined. In vitro translation of the cDNA resulted in a protein with an apparent molecular mass of about 60 kD.

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Human Sestrin-2 (SESN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SESN2. No significant cross-reactivity or interference between Human SESN2 and analogues was observed.

This Human Sestrin-2 (SESN2) ELISA Kit employs a two-site sandwich ELISA to quantitate SESN2 in samples. An antibody specific for SESN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySESN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SESN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SESN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.