Human Pulmonary surfactant-associated protein C (SP-C) ELISA Kit

SP-C is a fragment of a much larger precursor protein of 21 kD. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.Pulmonary surfactant-associated protein C is a membrane protein which manufactures surfactant.Humans and animals born lacking SP-C tend to develop progressive interstitial pneumonitis.The SFTPC gene encodes pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. It is produced exclusively by type II alveolar epithelial cells in the lung. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung.

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Human Pulmonary surfactant-associated protein C (SP-C) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFTPC. No significant cross-reactivity or interference between Human SFTPC and analogues was observed.

This Human Pulmonary surfactant-associated protein C (SP-C) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTPC in samples. An antibody specific for SFTPC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTPC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPC bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.