Human Sialic acid-binding Ig-like lectin 7 (SIGLEC7) ELISA Kit

The highest amino acid sequence similarity found was with the myeloid-specific CD33 molecule , and similar to other sialoadhesin molecules, AIRM1 appears to mediate sialic acid-dependent ligand recognition. By analyzing genomic DNA samples derived from a panel of human/hamster somatic cell hybrids, Falco et al. (1999) mapped the SIGLEC7 gene to chromosome 19. They noted that other members of the sialoadhesin family map to chromosome 19, including CD33, SIGLEC6, SIGLEC5, myelin-associated glycoprotein (MAG), and CD22 . Therefore, SIGLEC7 is probably located on 19q13.3, where these other family members have been localized. Human tissues show strong expression of Siglec-7 mRNA in spleen, peripheral blood leukocytes, and liver.

SIGLEC7; AIRM1; CD328; CDw328; D-siglec; QA79; SIGLEC-7; p75; p75/AIRM1; adhesion inhibitory receptor molecule 1; siglec-7; sialic acid binding immunoglobulin-like lectin 7

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Human Sialic acid-binding Ig-like lectin 7 (SIGLEC7) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIGLEC7. No significant cross-reactivity or interference between Human SIGLEC7 and analogues was observed.

This Human Sialic acid-binding Ig-like lectin 7 (SIGLEC7) ELISA Kit employs a two-site sandwich ELISA to quantitate SIGLEC7 in samples. An antibody specific for SIGLEC7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIGLEC7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

SIGLEC7; AIRM1; CD328; CDw328; D-siglec; QA79; SIGLEC-7; p75; p75/AIRM1; adhesion inhibitory receptor molecule 1; siglec-7; sialic acid binding immunoglobulin-like lectin 7

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.