Human Signal-regulatory protein beta-1 (SIRPB1) ELISA Kit

Signal-regulatory protein beta 1 is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Alternatively spliced transcript variants have been found for this gene. The signal regulatory protein-beta1 (SIRPbeta1) is a DAP12-associated transmembrane receptor expressed in a subset of hematopoietic cells. Recently, it was shown that peritoneal macrophages express SIRPbeta1, which positively regulated phagocytosis.

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Human Signal-regulatory protein beta-1 (SIRPB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIRPB1. No significant cross-reactivity or interference between Human SIRPB1 and analogues was observed.

This Human Signal-regulatory protein beta-1 (SIRPB1) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRPB1 in samples. An antibody specific for SIRPB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRPB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRPB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRPB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.