Human NAD-dependent deacetylase sirtuin-5 (SIRT5/SIR2L5) ELISA Kit

SIRT5 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.

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Human NAD-dependent deacetylase sirtuin-5 (SIRT5/SIR2L5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIRT5. No significant cross-reactivity or interference between Human SIRT5 and analogues was observed.

This Human NAD-dependent deacetylase sirtuin-5 (SIRT5/SIR2L5) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT5 in samples. An antibody specific for SIRT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.