Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit

S-phase kinase-associated protein 2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase.

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Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SKP2. No significant cross-reactivity or interference between Human SKP2 and analogues was observed.

This Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SKP2 in samples. An antibody specific for SKP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.