Human RAC-alpha serine/threonine-protein kinase (AKT1) ELISA Kit

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

AKT1; AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1 kinase; RAC-alpha serine/threonine-protein kinase; protein kinase B; rac protein kinase alpha

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human RAC-alpha serine/threonine-protein kinase (AKT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human AKT1. No significant cross-reactivity or interference between Human AKT1 and analogues was observed.

This Human RAC-alpha serine/threonine-protein kinase (AKT1) ELISA Kit employs a two-site sandwich ELISA to quantitate AKT1 in samples. An antibody specific for AKT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAKT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

AKT1; AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1 kinase; RAC-alpha serine/threonine-protein kinase; protein kinase B; rac protein kinase alpha

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.