Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit

The deduced 227-amino acid protein has a calculated molecular mass of 25.6 kD. AK3 shares 57.4% amino acid identity with AK4 (AK3L1) . It shares 75%, 90%, and 91% amino acid identity with bovine, rat, and mouse Ak3, respectively. Northern blot analysis detected a 3.2-kb transcript in all tissues examined. Expression was highest in heart, liver, and skeletal muscle, moderate in kidney and pancreas, and weak in brain, placenta, and lung. Transcripts of 5, 1.8, and 1.35 kb were detected in heart, liver, and pancreas. Western blot analysis detected AK3 at an apparent molecular mass of 28 kD in all tissues examined. Subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction.Human embryonic kidney cells had endogenous phosphotransferase activity, which increased following transfection of AK3 cDNA.

AK3; RP11-6J24.4; AK3L1; AK6; AKL3L; AKL3L1; FIX; adenylate kinase 3 alpha like; adenylate kinase 3 like 1; adenylate kinase 6; adenylate kinase 3 like 1

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit has high sensitivity and excellent specificity for detection of Human AK3. No significant cross-reactivity or interference between Human AK3 and analogues was observed.

This Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit employs a two-site sandwich ELISA to quantitate AK3 in samples. An antibody specific for AK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

AK3; RP11-6J24.4; AK3L1; AK6; AKL3L; AKL3L1; FIX; adenylate kinase 3 alpha like; adenylate kinase 3 like 1; adenylate kinase 6; adenylate kinase 3 like 1

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.