Human Natural resistance-associated macrophage protein 2 (SLC11A2) ELISA Kit

The SLC11A2 gene encodes a divalent metal transporter (DMT1), which carries iron, manganese, cobalt, nickel, cadmium, lead, copper, and zinc. DMT1 participates in cellular iron absorption at the luminal surface of the duodenum as well as in other areas of the body. Predicted amino acid sequence analysis of the NRAMP2 polypeptide identified a polytopic membrane protein highly homologous to human NRAMP1, with 66% identical residues (80% overall homology), resulting in identical predicted secondary structure of the 2 proteins. Sequence conservation is particularly high in the predicted transmembrane domains (90%), suggesting that these regions play a key role in the structural and functional aspects common to both proteins. As opposed to its NRAMP1 counterpart, whose expression is restricted to phagocytic cells.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Natural resistance-associated macrophage protein 2 (SLC11A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC11A2. No significant cross-reactivity or interference between Human SLC11A2 and analogues was observed.

This Human Natural resistance-associated macrophage protein 2 (SLC11A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC11A2 in samples. An antibody specific for SLC11A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC11A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC11A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC11A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.