Human Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit

The major reabsorptive mechanism for D-glucose in the kidney is known to involve a lower affinity, high capacity Na (+) /glucose cotransporter, which is located in the early proximal convoluted tubule segment S1 and has a Na (+) -to-glucose coupling ratio of 1:1.Uptake experiments using cRNA-injected Xenopus oocytes showed specific uptake of glucose and alpha-methyl glucopyranoside at up to three times background. This uptake was saturable, suggesting that the cDNA corresponds to a low-affinity kidney sodium-glucose transporter. It was therefore referred to as sodium-glucose cotransporter 2, or SGLT2. SGLT2 mediates saturable Na (+) -dependent and phlorizin-sensitive transport with K (m) values consistent with low affinity Na (+) /glucose cotransport.

SLC5A2; SGLT2; Na (+) /glucose cotransporter 2; low affinity sodium-glucose cotransporter; solute carrier family 5 (sodium/glucose transporter) ; member 2

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC5A2. No significant cross-reactivity or interference between Human SLC5A2 and analogues was observed.

This Human Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC5A2 in samples. An antibody specific for SLC5A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC5A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC5A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC5A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

SLC5A2; SGLT2; Na (+) /glucose cotransporter 2; low affinity sodium-glucose cotransporter; solute carrier family 5 (sodium/glucose transporter) ; member 2

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.