Human Asc-type amino acid transporter 1 (SLC7A10) ELISA Kit

SLC7A10, in association with 4F2HC (SLC3A2), mediates high-affinity transport of D-serine and several other neutral amino acids.The deduced 530-amino acid protein contains 12 transmembrane regions and a conserved cysteine residue (cys160) in the putative extracellular loop between transmembrane domains 3 and 4. This residue was presumed to be linked to 4f2hc via a disulfide bond. Northern blot analysis detected a 1.9-kb transcript in mouse brain and lung, with weaker expression in placenta. A single 4.4-kb transcript was expressed in small intestine.ASC1 shares 91% identity with mouse Asc1. Northern blot analysis detected 1.3-, 2.0-, 4.3-, and 6.0-kb transcripts in brain, heart, placenta, skeletal muscle, and kidney. The 2.0-kb transcript predominated in brain.

SLC7A10; FLJ20839; HASC-1; asc-1; asc-type amino acid transporter 1; solute carrier family 7; (cationic amino acid transporter; y+ system) member 10; solute carrier family 7; member 10

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Human Asc-type amino acid transporter 1 (SLC7A10) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC7A10. No significant cross-reactivity or interference between Human SLC7A10 and analogues was observed.

This Human Asc-type amino acid transporter 1 (SLC7A10) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC7A10 in samples. An antibody specific for SLC7A10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

SLC7A10; FLJ20839; HASC-1; asc-1; asc-type amino acid transporter 1; solute carrier family 7; (cationic amino acid transporter; y+ system) member 10; solute carrier family 7; member 10

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.