Human Sarcolipin (SLN) ELISA Kit

Sarcoplasmic reticulum Ca (2+) -ATPases are transmembrane proteins that catalyze the ATP-dependent transport of Ca (2+) from the cytosol into the lumen of the sarcoplasmic reticulum in muscle cells. Sarcolipin is a small proteolipid that regulates several sarcoplasmic reticulum Ca (2+) -ATPases. The transmembrane protein interacts with Ca (2+) -ATPases and reduces the accumulation of Ca (2+) in the sarcoplasmic reticulum without affecting the rate of ATP hydrolysis.SLN is highly expressed in rabbit fast-twitch skeletal muscle, but it is expressed to a lower extent in slow-twitch muscle and to an even lower extent in cardiac muscle, where ATP2A2 and PLN are highly expressed. It is expressed in only trace amounts in pancreas and prostate.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Sarcolipin (SLN) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLN. No significant cross-reactivity or interference between Human SLN and analogues was observed.

This Human Sarcolipin (SLN) ELISA Kit employs a two-site sandwich ELISA to quantitate SLN in samples. An antibody specific for SLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLN bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.