Human Acid sphingomyelinase (ASM) ELISA Kit

Sphingomyelin phosphodiesterase or simply Sphingomyelinase (SMase) is a hydrolase enzyme that is involved in sphingolipid metabolism reactions. SMase is a member of the DNase I superfamily of enzymes and is responsible for breaking sphingomyelin (SM) down into phosphocholine and ceramide. The activation of SMase has been suggested as a major route for the production of ceramide in response to cellular stresses. Sphingomyelin phosphodiesterase 1, acid lysosomal (acid sphingomyelinase), also known as SMPD1, is a human sphingomyelin phosphodiesterase. Defects in SMPD1 gene cause Niemann-Pick disease, SMPD1-associated.

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Human Acid sphingomyelinase (ASM) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMPD1. No significant cross-reactivity or interference between Human SMPD1 and analogues was observed.

This Human Acid sphingomyelinase (ASM) ELISA Kit employs a two-site sandwich ELISA to quantitate SMPD1 in samples. An antibody specific for SMPD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMPD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMPD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMPD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.