Human Sphingomyelin phosphodiesterase 2 (SMPD2) ELISA Kit

SMPD2 isa protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. Neutral sphingomyelinase (N-SMase) activity was first described in fibroblasts from patients with Niemann-Pick disease – a lysosomal storage disease characterized by deficiencies in acid SMase . Subsequent study found that this enzyme was the product of a distinct gene, had an optimum pH of 7.4, was dependent on Mg2+ ions for activity, and was particularly enriched in brain .

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Human Sphingomyelin phosphodiesterase 2 (SMPD2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMPD2. No significant cross-reactivity or interference between Human SMPD2 and analogues was observed.

This Human Sphingomyelin phosphodiesterase 2 (SMPD2) ELISA Kit employs a two-site sandwich ELISA to quantitate SMPD2 in samples. An antibody specific for SMPD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMPD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMPD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMPD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.