Human VPS10 domain-containing receptor SorCS1 (SORCS1) ELISA Kit

SORCS1 encodes one family member of vacuolar protein sorting 10 (VPS10) domain-containing receptor proteins. The VPS10 domain name comes from the yeast carboxypeptidase Y sorting receptor Vps10 protein. Members of this gene family are large with many exons but the CDS lengths are usually less than 3700 nt. Very large introns typically separate the exons encoding the VPS10 domain; the remaining exons are separated by much smaller-sized introns. These genes are strongly expressed in the central nervous system. Two of the five family members (sortilin and sortilin-related receptor) are synthesized as preproproteins; it is not yet known if this encoded protein is also a preproprotein. Alternatively spliced transcript variants encoding different isoforms have been identified.

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Human VPS10 domain-containing receptor SorCS1 (SORCS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SORCS1. No significant cross-reactivity or interference between Human SORCS1 and analogues was observed.

This Human VPS10 domain-containing receptor SorCS1 (SORCS1) ELISA Kit employs a two-site sandwich ELISA to quantitate SORCS1 in samples. An antibody specific for SORCS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySORCS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORCS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORCS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.