Human Transcription factor PU.1 (SPI1) ELISA Kit

Transcription factor PU.1 (SPI1) is an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. SPI1 can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for SPI1.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Transcription factor PU.1 (SPI1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPI1. No significant cross-reactivity or interference between Human SPI1 and analogues was observed.

This Human Transcription factor PU.1 (SPI1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPI1 in samples. An antibody specific for SPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.