Human Tyrosine hydroxylase (TH) ELISA Kit

Tyrosine hydroxylase (TH) is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in TH have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for TH.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Tyrosine hydroxylase (TH) ELISA Kit has high sensitivity and excellent specificity for detection of Human TH. No significant cross-reactivity or interference between Human TH and analogues was observed.

This Human Tyrosine hydroxylase (TH) ELISA Kit employs a two-site sandwich ELISA to quantitate TH in samples. An antibody specific for TH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.