Human Taste receptor type 2 member 19 (TAS2R19) ELISA Kit

TAS2R19 (Taste 2 Receptor Member 19) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Peptide ligand-binding receptors. GO annotations related to this gene include G-protein coupled receptor activity and bitter taste receptor activity. An important paralog of this gene is TAS2R46.

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Human Taste receptor type 2 member 19 (TAS2R19) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAS2R19. No significant cross-reactivity or interference between Human TAS2R19 and analogues was observed.

This Human Taste receptor type 2 member 19 (TAS2R19) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R19 in samples. An antibody specific for TAS2R19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS2R19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS2R19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS2R19 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.