Human E3 ubiquitin-protein ligase synoviolin (SYVN1) ELISA Kit

E3 ubiquitin-protein ligase synoviolin (SYVN1) involved in endoplasmic reticulum (ER) -associated degradation. E3 ubiquitin-protein ligase synoviolin removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. E3 ubiquitin-protein ligase synoviolin also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms.

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Human E3 ubiquitin-protein ligase synoviolin (SYVN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYVN1. No significant cross-reactivity or interference between Human SYVN1 and analogues was observed.

This Human E3 ubiquitin-protein ligase synoviolin (SYVN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYVN1 in samples. An antibody specific for SYVN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYVN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYVN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYVN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.