Human Synaptotagmin-like protein 4 (SYTL4) ELISA Kit

SYTL4 encodes a member of the synaptotagmin like protein family. Members of this family are characterized by an N-terminal Rab27 binding domain and C-terminal tandem C2 domains. Synaptotagmin-like protein 4 binds specific small Rab GTPases and is involved in intracellular membrane trafficking. This protein binds Rab27 and may be involved in inhibiting dense core vesicle exocytosis. Alternate splicing results in multiple transcript variants that encode the same protein.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Synaptotagmin-like protein 4 (SYTL4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYTL4. No significant cross-reactivity or interference between Human SYTL4 and analogues was observed.

This Human Synaptotagmin-like protein 4 (SYTL4) ELISA Kit employs a two-site sandwich ELISA to quantitate SYTL4 in samples. An antibody specific for SYTL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYTL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYTL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYTL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.