Human Synaptotagmin-8 (SYT8) ELISA Kit

SYT8 encodes a member of the synaptotagmin protein family. Synaptotagmins are membrane proteins that are important in neurotransmission and hormone secretion, both of which involve regulated exocytosis. Expression of Synaptotagmin-8 in human pancreatic islets has been connected to activity of the promoter for the insulin gene, on the same chromosome several hundred kilobases away. This association would link response to gluclose to insulin secretion. Alternative splicing results in multiple transcript variants encoding different isoforms.

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Human Synaptotagmin-8 (SYT8) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYT8. No significant cross-reactivity or interference between Human SYT8 and analogues was observed.

This Human Synaptotagmin-8 (SYT8) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT8 in samples. An antibody specific for SYT8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.