Human Synaptotagmin-5 (SYT5) ELISA Kit

Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Synaptotagmin-5 (SYT5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYT5. No significant cross-reactivity or interference between Human SYT5 and analogues was observed.

This Human Synaptotagmin-5 (SYT5) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT5 in samples. An antibody specific for SYT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.