Human Synaptotagmin-12 (SYT12) ELISA Kit

Syt12 colocalises with and binds Syt1 on synaptic vesicles, but regulates spontaneous release independently from Syt1. Syt12 is phosphorylated by cAMP-dependent protein kinase A (PKA) at a single site, and mutation of this site blocks the effect of Syt12 on spontaneous release. Syt12 is not essential for basal synaptic transmission, but is required for cAMP-dependent mossy fibre long-term potentiation (LTP), a presynaptic form of long-term plasticity that is induced by PKA activation

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Human Synaptotagmin-12 (SYT12) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYT12. No significant cross-reactivity or interference between Human SYT12 and analogues was observed.

This Human Synaptotagmin-12 (SYT12) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT12 in samples. An antibody specific for SYT12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.