Human Synaptojanin-2 (SYNJ2) ELISA Kit

Synaptojanin-2 is a ubiquitously expressed inositol polyphosphate 5-phosphatase. The longest deduced protein, designated Synj2-alpha, contains 1,216 amino acids and has a calculated molecular mass of 140 kD. Synj2 contains an N-terminal Sac1 (SACM1L) homology domain, a central inositol 5-prime-phosphatase domain, and a C-terminal proline-rich region. Synj2 variants arise from the use of 2 possible initiation sites and at least 6 different exons encoding C-terminal domains. Initiation from the alternative start sites results in isoforms that vary in the presence of 84 amino acids in the N-terminal part of the Sac1 homology domain. Database analysis indicated the presence of a third putative initiation site in human SYNJ2, resulting in a protein that lacks most of the Sac1 homology domain.

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Human Synaptojanin-2 (SYNJ2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYNJ2. No significant cross-reactivity or interference between Human SYNJ2 and analogues was observed.

This Human Synaptojanin-2 (SYNJ2) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNJ2 in samples. An antibody specific for SYNJ2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNJ2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNJ2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNJ2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.