Human Synaptonemal complex protein 3 (SYCP3) ELISA Kit

SYCP3 encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. Three meiosis-specific components of the SC have been characterized in mammals, SC protein-1 (SYCP1), SYCP2, and SYCP3. SYCP3 has a molecular mass of 30 kD and a C-terminal coiled-coil domain that has been shown to promote homotypic interactions in vitro; the SYCP3 gene has been cloned in mouse, rat, and hamster.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Synaptonemal complex protein 3 (SYCP3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYCP3. No significant cross-reactivity or interference between Human SYCP3 and analogues was observed.

This Human Synaptonemal complex protein 3 (SYCP3) ELISA Kit employs a two-site sandwich ELISA to quantitate SYCP3 in samples. An antibody specific for SYCP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYCP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYCP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYCP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.