Human Small ubiquitin-related modifier 2 (SUMO2) ELISA Kit

Small ubiquitin-related modifier 2 is a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

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Human Small ubiquitin-related modifier 2 (SUMO2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SUMO2. No significant cross-reactivity or interference between Human SUMO2 and analogues was observed.

This Human Small ubiquitin-related modifier 2 (SUMO2) ELISA Kit employs a two-site sandwich ELISA to quantitate SUMO2 in samples. An antibody specific for SUMO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySUMO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SUMO2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SUMO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.