Human Sulfatase-modifying factor 1 (SUMF1) ELISA Kit

Sulfatases catalyze the hydrolysis of sulfate esters such as glycosaminoglycans, sulfolipids, and steroid sulfates. C-alpha-formylglycine (FGly), the catalytic residue in the active site of eukaryotic sulfatases, is posttranslationally generated from a cysteine by SUMF1, the FGly-generating enzyme (FGE), in the endoplasmic reticulum (ER) . The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in multiple sulfatase deficiency (MSD), a lysosomal storage disorder (Roeser et al., 2006) .Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Sulfatase-modifying factor 1 (SUMF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SUMF1. No significant cross-reactivity or interference between Human SUMF1 and analogues was observed.

This Human Sulfatase-modifying factor 1 (SUMF1) ELISA Kit employs a two-site sandwich ELISA to quantitate SUMF1 in samples. An antibody specific for SUMF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySUMF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SUMF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SUMF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.