Human Striatin (STRN) ELISA Kit

Striatin, a quantitatively minor protein belonging to the WD-repeat family of proteins, is a Ca2+/calmodulin-binding protein mostly expressed in the striatum and in the motor and olfactory systems. Generally associated with membranes, striatin is mostly found in dendritic spines where it is likely to play a role in Ca2+-signaling events. The predicted corresponding peptide is potentially able to form a basic amphiphilic helix, as is often the case for many known calmodulin-binding sites. Calmodulin binding to striatin is Ca2+-dependent, with half-maximal binding occurring around 0.5 μm free Ca2+. In the presence of Ca2+, the equilibrium dissociation constant of calmodulin/striatin fusion protein complex is 40 ± 5 nm.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Striatin (STRN) ELISA Kit has high sensitivity and excellent specificity for detection of Human STRN. No significant cross-reactivity or interference between Human STRN and analogues was observed.

This Human Striatin (STRN) ELISA Kit employs a two-site sandwich ELISA to quantitate STRN in samples. An antibody specific for STRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTRN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STRN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STRN bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.